Fusion: Practice and Applications FPA 2692-4048 2770-0070 10.54216/FPA https://www.americaspg.com/journals/show/1050 2018 2018 Faculty of Engineering, Fayoum University, Fayoum , Egypt Osama Osama problem as it requires rebuilding the original sequence of DNA from a massive number of fragments. This paper introduces an efficient hybridization between Harris Hawks Optimization (HHO) and Problem Aware Local Search (PALS) to be utilized in solving DNA Fragment Assembly Problem (FAP). The efficiency of the proposed hybrid algor In computational molecular biology, the sequencing of Deoxyribonucleic Acid (DNA) is a very challenging ithm (PHHO) is compared with the original PALS, Firefly Algorithm (FA), Genetic Algorithm (GA), Artificial Bee Colony (ABC), and GAG50. The experimental results show the efficiency of the proposed algorithm compared to other approaches. 2022 2022 66 78 10.54216/FPA.070201 https://www.americaspg.com/articleinfo/3/show/1050