aspg-41-3364-1792223310 20261017074830 American Scientific Publishing Group admin@americaspg.com American Scientific Publishing Group Metaheuristic Optimization Review MOR 3066-280X 2024 1 1 Nima Khodadadi Department of Civil and Architectural Engineering, University of Miami, Coral Gables, FL, USA Benyamin Abdollahzadeh Department of Mathematics, Faculty of Science, University of Hradec Králové, 500 03, Hradec Králové, Czech Republic Following this background, this review discusses the advanced technologies such as AI, micro-fluids, and automated platforms that this differentiation protocol could help achieve in regenerative medicine. Stem cell research, essential in tissue engineering, disease modeling, and drug development, is challenging through heterogeneity, scalability, and reproducibility, as observed in differentiation procedures. Machine learning and deep learning patterns have become more effective in predicting cellular behavior, tracking cellular stations, and optimizing differentiation methods for current stem cell technology. These methods also reduce observer bias, increase the throughput of high-throughput screening, and advance modeling to precise therapeutic applications. At the same time, microfluidic and automated systems provide nearly perfect control over differentiation stimuli, recreating the in vivo conditions with the ability to control spatial and temporal gradients. This integration between AI and microengineering has promoted 3D culture systems, lab-on-a-chip technologies, and biosensors, enabling reproducible and efficient differentiation results. There is still much to accomplish, such as the problem of obtaining uniform stem cell populations or decoding the tissue context. This field incorporates several interdisciplinary advancements such as stimuli-responsive systems and computational modeling; it envisages new horizons in regenerative medicine, transforming stem cell-based therapeutic technologies to their optimum level for personalized medicine and other advanced tissue engineering applications. 2024 17 34 3364 https://creativecommons.org/licenses/by/4.0/ 10.54216/MOR.010102 https://americaspg.com/journal/41/article/3364 https://americaspg.com/storage/01733575393.pdf